lentiviral transfer vector (Addgene inc)

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Addgene inc lentiviral transfer vector
Lentiviral Transfer Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 15515 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+transfer+vectors/psPAX2+(Plasmid+%2312260)/pm41597202-77-4-10
Average 98 stars, based on 15515 article reviews

lentiviral transfer vector - by Bioz Stars, 2026-10

98/100 stars

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Plasmid Preparation:

Article Title: Y-27632 acts beyond ROCK inhibition to maintain epidermal stem-like cells in culture.
Article Snippet: .. Lentiviral transfer vectors were co-transfected with psPAX2 (Addgene plasmid 12260, deposited by Didier Trono) as the packaging plasmid and pVSV-G (Addgene plasmid 8454, deposited by Bob Weinberg) as the envelope plasmid. ..

Article Title: Distinct genetic pathways define pre-malignant versus compensatory clonal hematopoiesis in Shwachman-Diamond syndrome.
Article Snippet: .. Lentiviral vector supernatants were generated by cotransfecting lentiviral transfer vectors (pRRLSFFV-gwdest, pLIX-403 (Addgene plasmid #41395), pLX304 (Addgene plasmid without improving the underlying ribosomal abnormalities, and promote the development of myeloid malignancies. .. NATURE COMMUNICATIONS | (2021) 12:1334 | https://doi.org/10.1038/s41467-021-21588-4 |www.nature.com/naturecommunications 11 #25890), tet-pLKO-puro (Addgene plasmid #21915), SMARTvector-humanshEIF6 (Dharmacon V3SH11243-07EG3692), SMARTvector-hCMV-shTP53TurboGFP (Dharmacon V3SH11243-00EG7157), SMARTvector-hCMV-shTP53TurboRFP (Dharmacon V3SH11243-07EG7157), pL40C.SFFV.eGFP.miR30N.

Article Title: Orgo-Seq integrates single-cell and bulk transcriptomic data to identify cell type specific-driver genes associated with autism spectrum disorder
Article Snippet: .. Lentiviruses were made in the HEK293T cells by co-transfection with VSV-G envelope expressing plasmid (pMD2.G addgene #12259), packaging plasmid (pCMV-dR8.2 dvpr #8455), and lentiviral transfer vectors (FUW-M2rtTA addgene #20342 and pTet-O-Ngn2-puro addgene #52047) using Lipofectamine 3000 reagents. .. Lentiviruses were harvested with the medium 48 h after transfection, pelleted by centrifugation (1,500 × g for 45 min) with Lenti-X Concentrator (Clontech), resuspended in DPBS, aliquoted, snap-frozen in liquid nitrogen, and stored in −80 °C.

Article Title: Y-27632 acts beyond ROCK inhibition to maintain epidermal stem-like cells in culture
Article Snippet: .. Lentiviral transfer vectors were co-transfected with psPAX2 (Addgene plasmid 12260 , deposited by Didier Trono) as the packaging plasmid and pVSV-G (Addgene plasmid 8454 , deposited by Bob Weinberg) as the envelope plasmid. ..

Generated:

Expressing:

Retroviral:

Article Title: RNA sensing via the RIG‐I‐like receptor LGP2 is essential for the induction of a type I IFN response in ADAR1 deficiency
Article Snippet: .. The above‐mentioned retroviral and lentiviral transfer vectors were combined with psPAX2 (Addgene, 12260; gift from Didier Trono) and pMD2.G (Addgene, 12259; gift from Didier Trono) at a 4:3:1 ratio and transfected using polyethylenimine (PEI; 23966, Polysciences). ..

Transfection:

Article Title: Engineering of human mesenchymal stem cells resistant to multiple natural killer subtypes
Article Snippet: .. 293T cells were transfected with lentiviral transfer vectors, pCMVR8.74 (Addgene #22036, a gift of Didier Trono), and pMD2.G (Addgene #12259, a gift of Didier Trono) at a ratio of 3:2:1 using the Lipofectamine 3000 transfection reagent (Thermo, L30000015). ..

other:

Article Title: Distinct genetic pathways define pre-malignant versus compensatory clonal hematopoiesis in Shwachman-Diamond syndrome
Article Snippet: One milliliter was plated in triplicate wells of six-well Smartdishes (Stem Cell Technologies, 27370).

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